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Movie Gallery
Nucleotide excision repair of a lesion-containing DNA
A bulky DNA lesion derived from a highly potent polycyclic aromatic tumorigen stabilizes nucleosome core particle structure. Biochemistry. 2010 Nov 23;49(46):9943-5. doi: 10.1021/bi101560y
Cai Y, Wang L, Ding S, Schwaid A, Geacintov NE, Broyde S. PubMed Link
Differences in the Access of Lesions to the Nucleotide Excision Repair Machinery in Nucleosomes. Biochemistry. 2015 Jul 14;54(27):4181-5. doi: 10.1021/acs.biochem.5b00564.
Cai Y, Kropachev K, Terzidis MA, Masi A, Chatgilialoglu C, Shafirovich V, Geacintov NE, Broyde S. PubMed Link
(Left) Free energy profiles of base flipping in intercalative polycyclic aromatic hydrocarbon-damaged DNA duplexes: Energetic and structural relationships to nucleotide excision repair. Chem. Res. Toxicol. 2013 26(7):1115-25
Cai Y, Zheng H, Ding S, Kropachev K,Schwaid AG,Tang Y, Mu H, Wang S, Geacintov NE, Zhang Y, Broyde S. PubMed Link
(Right) Ribonucleotides as nucleotide excision repair substrates. DNA Repair (Amst). 2014 Jan;13:55-60. doi: 10.1016/j.dnarep.2013.10.010.
Cai Y, Geacintov NE, Broyde S. PubMed Link
Nucleotide excision repair of 2-acetylaminofluorene- and 2-aminofluorene-(C8)-guanine adducts: molecular dynamics simulations elucidate how lesion structure and base sequence context impact repair efficiencies.Nucleic Acids Res. 2012 Oct 40(19):9675-90
Mu H, Kropachev K, Wang L, Zhang L, Kolbanovskiy A, Kolbanovskiy M, E Geacintov N, Broyde S. PubMed Link
Differential nucleotide excision repair susceptibility of bulky DNA adducts in different sequence contexts: hierarchies of recognition signals. J Mol Biol. 2009 Jan 9;385(1):30-44. doi: 10.1016/j.jmb.2008.09.087.
Cai Y, Patel DJ, Geacintov NE, Broyde S. PubMed Link
Dynamics of a benzo[a]pyrene-derived guanine DNA lesion in TGT and CGC sequence contexts: Enhanced mobility in TGT explains conformational heterogeneity, flexible bending and greater susceptibility to nucleotide excision repair. J Mol Biol. 2007 Nov 23;374(2):292-305.
Cai Y, Patel DJ, Geacintov NE, Broyde S. PubMed Link
Nucleotide excision repair efficiencies of bulky carcinogen-DNA adducts are governed by a balance between stabilizing and destabilizing interactions.Biochemistry. 2012 Feb 21;51(7):1486-99. doi: 10.1021/bi201794x.
Cai Y, Geacintov NE, Broyde S. PubMed Link
G*CT* Cross-link
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(Left) Structural, energetic and dynamic properties of guanine(C8)-thymine(N3) cross-links in DNA provide insights on susceptibility to nucleotide excision repair. Nucleic Acids Res. 2012 40(6)2506-17.
S. Ding, K. Kropachev, Y. Cai, M. Kolbanovskiy, S. Durandina, Z. Liu, V. Shafirovich, S. Broyde and N. Geacintov PubMed Link
(Right) Base flipping free energy profiles for damaged and undamaged DNA. Chem.Res.Toxicol. 2010 23(12)1868-70.
Zheng H, Cai Y, Ding S, Tang Y, Kropachev K, Zhou Y, Wang L, Wang S, Geacintov NE, Zhang Y, Broyde S. PubMed Link
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(Left) Preferred WMSA catalytic mechanism of the nucleotidyl transfer reaction in human DNA polymerase k elucidates error-free bypass of a bulky DNA lesion.Nucleic Acids Res. 2012 Oct 1;40(18):9193-9205.
Lior-Hoffmann L, Wang L, Wang S. Geacintov NE, Broyde S, Zhang Y. PubMed Link
(Right) The N-clasp of human DNA polymerase kappa promotes blockage or error-free bypass of adenine- or guanine-benzo[a]pyrenyl lesions. Chem.Res.Toxicol. 2010 23(12)1868-70.
Jia L, Geacintov NE, Broyde S. PubMed Link
(Left) Resistance of bulky DNA lesions to nucleotide excision repair can result from extensive aromatic lesion-base stacking interactions. Nucleic Acids Res. 2011 39(20): 8752-64.
Reeves DA, Mu H, Kropachev K, Cai Y, Ding S, Kolbanovskiy A, Kolbanovskiy M, Chen Y, Krzeminski J, Amin S, Patel DJ, Broyde S, Geacintov NE. PubMed Link
(Right)Nucleotide excision repair efficiencies of bulky carcinogen-DNA adducts are governed by a balance between stabilizing and destabilizing interactions. Biochemistry. 2012 Feb 21;51(7):1486-99.
Cai Y, Geacintov NE, Broyde S. PubMed Link